ENTtoday
  • Home
  • COVID-19
  • Practice Focus
    • Allergy
    • Facial Plastic/Reconstructive
    • Head and Neck
    • Laryngology
    • Otology/Neurotology
    • Pediatric
    • Rhinology
    • Sleep Medicine
  • Departments
    • Issue Archive
    • TRIO Best Practices
      • Allergy
      • Facial Plastic/Reconstructive
      • Head and Neck
      • Laryngology
      • Otology/Neurotology
      • Pediatric
      • Rhinology
      • Sleep Medicine
    • Career Development
    • Case of the Month
    • Everyday Ethics
    • Health Policy
    • Legal Matters
    • Letter From the Editor
    • Medical Education
    • Online Exclusives
    • Practice Management
    • Resident Focus
    • Rx: Wellness
    • Special Reports
    • Tech Talk
    • Viewpoint
    • What’s Your O.R. Playlist?
  • Literature Reviews
    • Allergy
    • Facial Plastic/Reconstructive
    • Head and Neck
    • Laryngology
    • Otology/Neurotology
    • Pediatric
    • Rhinology
    • Sleep Medicine
  • Events
    • Featured Events
    • TRIO Meetings
  • Contact Us
    • About Us
    • Editorial Board
    • Triological Society
    • Advertising Staff
    • Subscribe
  • Advertise
    • Place an Ad
    • Classifieds
    • Rate Card
  • Search

Biofilms Likely Play Key Role in Pediatric Otitis Media and Otorrhea

by Amy E. Hamaker • August 9, 2016

  • Tweet
  • Email
Print-Friendly Version

What role do biofilms play in chronic and recurrent otitis media and otorrhea?

Bottom line: Positive labeling of otorrhea solids for extracellular DNA (eDNA) and integration host factor (IHF), in combination with microbiological culture results, indicated that biofilms likely play a key role in chronic otorrhea. DNABII proteins in association with eDNA, may serve as a key therapeutic target in post-tympanostomy tube otorrhea.

You Might Also Like

  • Alloiococcus otitidis May Play Large Role in Otits Media with Effusion
  • What is the Role of Tympanostomy Tubes in the Treatment of Recurrent Acute Otitis Media?
  • Ventilation Tubes in Otitis Media May Lower Quality of Life
  • Biofilms in Chronic Rhinosinusitis and Otitis Media
Explore This Issue
August 2016

Background: Otitis media with effusion and recurrent acute otitis media are common problems in children. Treatment commonly consists of systemic antibiotics, with surgical tympanostomy tube placement for those resistant to medical treatment; however, tympanostomy tubes and middle ear mucosa represent ideal environments for attachment of bacteria and biofilm growth. Biofilm formation has been investigated as a possible source of chronic otitis media and post-tube insertion otorrhea.

Setting: Department of Otolaryngology–Head and Neck Surgery, Nationwide Children’s Hospital, Columbus, Ohio.

Synopsis: A total of 15 samples were collected and processed for immunofluorescent labeling for the presence of a DNABII protein (e.g., IHF) and counterstained for extracellular DNA (eDNA). Of the 15 total specimens, 11 were culture-positive for bacterial species, with one yielding yeast. In four of 11 specimens that were culture-positive for bacterial species, one of the three predominant otopathogens of otitis media was cultured (e.g., nontypeable Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis). Two additional cultures yielded methicillin-resistant Staphylococcus aureus. The remaining specimens were of mixed microbial origin, while three specimens were culture-negative. Of the 15 samples labeled with rabbit anti-IHFE. coli and counterstained with DAPI, nine were positive for both eDNA and the DNA-binding protein IHF. Sixty percent of all specimens were positive for IHF, and eight of the 11 samples that were positive for microbial growth were also positive for labeling with antibodies directed against the DNABII protein IHF.

Citation: Idicula WK, Jurcisek JA, Cass ND, et al. Identification of biofilms in post-tympanostomy tube otorrhea. Laryngoscope. 2016;126: 1946-1951.

Filed Under: Literature Reviews, Otology/Neurotology, Otology/Neurotology, Pediatric, Pediatric, Practice Focus Tagged With: biofilm, ear infection, otitis media, otorrhea, pediatricsIssue: August 2016

You Might Also Like:

  • Alloiococcus otitidis May Play Large Role in Otits Media with Effusion
  • What is the Role of Tympanostomy Tubes in the Treatment of Recurrent Acute Otitis Media?
  • Ventilation Tubes in Otitis Media May Lower Quality of Life
  • Biofilms in Chronic Rhinosinusitis and Otitis Media

The Triological SocietyENTtoday is a publication of The Triological Society.

The Laryngoscope
Ensure you have all the latest research at your fingertips; Subscribe to The Laryngoscope today!

Laryngoscope Investigative Otolaryngology
Open access journal in otolaryngology – head and neck surgery is currently accepting submissions.

Classifieds

View the classified ads »

TRIO Best Practices

View the TRIO Best Practices »

Top Articles for Residents

  • Do Training Programs Give Otolaryngology Residents the Necessary Tools to Do Productive Research?
  • Why More MDs, Medical Residents Are Choosing to Pursue Additional Academic Degrees
  • What Physicians Need to Know about Investing Before Hiring a Financial Advisor
  • Tips to Help You Regain Your Sense of Self
  • Should USMLE Step 1 Change from Numeric Score to Pass/Fail?
  • Popular this Week
  • Most Popular
  • Most Recent
    • The Dramatic Rise in Tongue Tie and Lip Tie Treatment
    • Vertigo in the Elderly: What Does It Mean?
    • Experts Delve into Treatment Options for Laryngopharyngeal Reflux
    • Some Laryngopharyngeal Reflux Resists PPI Treatment
    • Weaning Patients Off of PPIs
    • New Developments in the Management of Eustachian Tube Dysfunction
    • Vertigo in the Elderly: What Does It Mean?
    • Some Laryngopharyngeal Reflux Resists PPI Treatment
    • Eustachian Tuboplasty: A Potential New Option for Chronic Tube Dysfunction and Patulous Disease
    • The Dramatic Rise in Tongue Tie and Lip Tie Treatment
    • Podcasts Becoming More Popular Method of Education for Otolaryngologists
    • How to Embrace Optimism in the Midst of the COVID-19 Pandemic
    • Tips on How to Approach Conversations with Patients about the COVID-19 Vaccine
    • Steps You Should Take to Protect Your Voice and Hearing During Telemedicine Sessions
    • Routine Postoperative Adjunct Treatments Unnecessary for Idiopathic Cerebrospinal Fluid Leaks

Polls

Have you spoken with your patients about receiving the COVID-19 vaccine?

View Results

Loading ... Loading ...
  • Polls Archive
  • Home
  • Contact Us
  • Advertise
  • Privacy Policy
  • Terms of Use

Visit: The Triological Society • The Laryngoscope • Laryngoscope Investigative Otolaryngology

Wiley
© 2021 The Triological Society. All Rights Reserved.
ISSN 1559-4939

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.